HSPG 2 ( heparan sulfate proteoglycan 2 )
نویسندگان
چکیده
The transcribed mRNA is 14,294 bp (Cohen et al., 1993). An alternative transcript for unc-52, the homologous gene to perlecan in C. elegans, has been reported (Spike et al., 2002). In addition, a human alternative transcript was submitted to the NCBI (Accession Q8TEU3_HUMAN) as a sequence for a short version variant, miniperl, encoding a 240 amino acid protein of 25.942 kD. Expression of HSPG2 was found to be induced by TGF-beta via NF-1 (Iozzo et al., 1997), and inhibited by NF-gamma via STAT1 (Sharma and Iozzo, 1998).
منابع مشابه
Heparan sulfate proteoglycans from mouse mammary epithelial cells: localization on the cell surface with a monoclonal antibody
Mouse mammary epithelial cells, of the normal murine mammary gland (NMuMG) cell line, bear a heparan sulfate-rich proteoglycan (HSPG) on their surfaces. A hybridoma (281-2) secreting a monoclonal antibody that recognizes this HSPG was produced by fusion of SP-2/0 myeloma cells with spleen cells from rats immunized with NMuMG cells. The 281-2 monoclonal antibody is directed against the core prot...
متن کاملHeparan sulfate proteoglycan and laminin mediate two different types of neurite outgrowth.
Spinal cord neurons cultured in vitro have been shown to respond to changes in their environment by means of 2 different types of neurite outgrowth: (1) neurite elongation and (2) emergence and branching of newly formed neurites. Culture of spinal cord neurons with heparan sulfate proteoglycan (HSPG) medium resulted in a 3-fold increase in neurite elongation compared to the control. Extensive b...
متن کاملHeterogeneous distribution of a basement membrane heparan sulfate proteoglycan in rat tissues
A heparan sulfate proteoglycan (HSPG) synthesized by murine parietal yolk sac (PYS-2) cells has been characterized and purified from culture supernatants. A monospecific polyclonal antiserum was raised against it which showed activity against the HSPG core protein and basement membrane specificity in immunohistochemical studies on frozen tissue sections from many rat organs. However, there was ...
متن کاملHypoxia increases macrophage motility, possibly by decreasing the heparan sulfate proteoglycan biosynthesis.
Macrophages are recruited and retained in hypoxic sites in atherosclerotic lesions and tumors. Furthermore, macrophages are suggested to be a major source of HSPG synthesis in atherosclerotic lesions. HSPG are, among other things, known to regulate cell motility, cell adhesion, and receptor interaction. The aim of this study was to investigate the effect of hypoxia on HSPG expression and macrop...
متن کاملHeparan sulfate proteoglycan from the extracellular matrix of human lung fibroblasts. Isolation, purification, and core protein characterization.
Confluent cultured human lung fibroblasts were labeled with 35SO4(2-). After 48 h of labeling, the pericellular matrix was prepared by Triton X-100 and deoxycholate extraction of the monolayers. Heparan sulfate proteoglycan (HSPG) accounted for nearly 80% of the total matrix [35S]proteoglycans. After solubilization in 6 M guanidinium HCl and cesium chloride density gradient centrifugation, the ...
متن کاملIdentification of a cell surface-binding protein for the core protein of the basement membrane proteoglycan.
We have identified a protein(s) on the surface of hepatocytes that binds to the core protein of the heparan sulfate proteoglycan of basement membranes. These cells attached and spread on substrates prepared from the basement membrane heparan sulfate proteoglycan (HSPG) and its core protein (HSPG-core). Three proteins (Mr = 38,000, 36,000, and 26,000) were found to bind to a HSPG-core affinity c...
متن کامل